2.7 Evaluation of canine leishmaniasis vaccine under field conditions in Spain

Charles University in Prague (CUNI), - Barcelona Institute for Global Health (ISGlobal), Barcelona, Spain   - Clínica Veterinaria CANIS, Girona, Spain   - Universitat de Barcelona (UB), Barcelona, Spain

Area of research: Prevention
Research programme: Implementation

Objective:

To evaluate a vaccine against canine leishmaniasis (CanL) under field conditions in Spain to monitoring the control of the disease.

Expected results:

(1) The utility of dog’s vaccination against canine leishmaniasis in rural population living in optimal conditions for transmission.

(2) The possible existence of cross-reactions by serological test after vaccination that may disturb in the epidemiological studies on CanL.

(3) The correlation between CanL infection and success or failure of vaccination and the exposition to the vector in natural conditions for Leishmania transmission 

Summary:

Background: In recent years, Leishmania vectors as well as cases of CanL have been observed in new areas in Spain, France, Italy and Portugal, and the number of cases has also increased in some endemic areas In order to control the disease, several methods have been postulated including immunization of animals. To our knowledge, there are currently three vaccines in the market for this purpose, two in Brazil and one in Europe. Although there are data on the evaluation of vaccines in dogs in natural conditions in Brazil, no data are available from independent studies in Europe where the vaccine is commercialized, including Spain.

Methodology: 1) To identify areas of CanL. 2) To vaccinate dogs from rural areas and whose owners request following the recommendations of the manufacturer (CaniLeish®). 3) To carry out an epidemiological survey on CanL of the nearest areas of the vaccinated dogs. 4) To study the ratio of parasitized Phlebotomus females in the places of vaccinated dogs. 5) To monitor vaccinated dogs (serology, PCR, culture) to separate vaccinated from naturally infected, and 6) To test a new diagnostic test (developed in CUNI) to see the exposition of dogs to P. perniciosus.